Monolayer culturing of human gingival fibroblasts on different titanium surfaces. 2005 (English)Independent thesis Basic level (degree of Bachelor)Student thesis

Abstract [en]

In all time people have lost their teeth for different reasons. When installing an implant, the interaction between the oral mucosa and the implant should be strong enough to prevent influences on the microbiota. Human gingival fibroblasts are the predominant connective tissue cells and are involved in the production of collagen, which is an important protein in the adhesion to the implant. The aims of this investigation was to answer the question: Are there differences in culturing HGF on untreated or sandpapered titanium surfaces, when it comes to the growth, proliferation and the direction that the cells grow in? The aims were also to study the different surfaces on the titanium coins that are used in this investigation, with a SEM and to investigate which is the most effective method to stain and fixate HGF. HGF were cultured on different titanium surfaces to be counted and stained. This study showed no significant difference between the growths of HGF cultured on smooth and rough titanium surfaces, however there was a tendency that the cells preferred the rough surface to the smooth. The combination of methanol and FITC as fixation and staining methods, gave the sharpest and the most detailed pictures. This combination was therefore chosen when staining HGF on the titanium coins. The staining of the HGF on the titanium coins clearly showed that HGF grew in different patterns on the different surfaces. The pictures taken with SEM showed peaks and valleys on the sandpapered surfaces and some irregularities on the untreated surface.

Place, publisher, year, edition, pages

2005.

Keywords [en]

human gingival fibroblasts, titanium

Identifiers

URN: urn:nbn:se:hh:diva-13023Local ID: U12093OAI: oai:DiVA.org:hh-13023DiVA, id: diva2:368192

Uppsok

Life Earth Science

Note

Denna uppsats kan beställas från arkivet / This paper can be ordered from the archive. Kontakta / Contact: arkivet@hh.seAvailable from: 2010-11-09 Created: 2010-11-09 Last updated: 2025-10-01Bibliographically approved

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