In recent decades, the negative effects of artificial light at night on natural ecosystems have attracted the attention of ecologists. Studies have shown that artificial light at night leads to a considerable reduction in insects and has a worrying impact on terrestrial ecosystems, including nocturnal insects (e.g. Lepidoptera) such as moths. Warm white light is generally expected to have a lower ecological impact compared with cold white light which has a higher proportion of blue light (< 500 nm). The aim of this study is to investigate the impact of three light emitting diode (LED) light sources with different spectral power distributions on the responses of the greater wax moth (Galleria mellonella) under controlled experimental settings. In this experiment, three light sources with different spectral power distribution and dark condition were used and the start response time, time active, time flap wings, time flap wings / time active, main activity area and stop area of the greater wax moth were investigated. The light treatment used were: (1) darkness (n = 13); (2) warm-white light (correlated color temperature of 2675 K, n = 12); (3) white light (4070 K, n = 4); (4) cold white light (6200 K, n = 8). The experiment was performed in a rectangular light-tight box under controlled conditions. Main activity area showed significant difference between warm-white light and cold white light. With cold white light, a larger proportion of the moths were active in the area with the highest light levels, while the main activity area in the warm white light was in the zone with the lowest light levels. Other variables, however, did not show significant differences. The conclusion is that warm white light had a lower ecological impact than cold white light due to a larger proportion of moth is attracted to areas with stronger light. The results support the notion that in outdoor environments, warm white LEDs (maximum 3000 K) should be preferred compared to cold white light LEDs (over 3000 K), to reduce the impact on insects such as moths. The low correlated color temperature light sources used outdoors deserves more in-depth development and research.