Climate and land use changes can increase terrestrial runoff to aquatic systems, leading to brownification and eutrophication in northern boreal lakes. Brownification may boost bacterial respiration and production, while eutrophication can enhance primary production and algal blooms. However, their combined effects on basal producers and bacterial carbon utilization are less understood. This study explores the combined impacts of the two stressors: brownification and eutrophication on microbial dynamics in Lake Bolmen. Utilizing a field mesocosm experimental design, treatments received different combinations of organic matter (OM) and inorganic nutrients to simulate predicted future scenarios. Results showed that OM additions significantly increased bacterial production and respiration, regardless of nutrient additions. Nutrient additions enhanced bacterial production but did not affect respiration. Both nutrients and OM stimulated bacterial growth efficiency. Labile carbon from DOM was the main driver of higher bacterial respiration and short-term production increases. Fluorescence data indicated that the combination of brownification and eutrophication led to higher terrestrial DOM utilization than each stressor alone. The study suggests that future boreal lakes may become more heterotrophic, thus increasing CO2 release. These findings highlight the complex interactions between DOM and nutrients and underscore the importance of considering multiple stressors in lake management and mitigation strategies. © The Author(s) 2024