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  • 1.
    Burestedt, E.
    et al.
    Department of Analytical Chemistry, University of Lund, Lund, Sweden.
    Emnéus, J.
    Department of Analytical Chemistry, University of Lund, Lund, Sweden.
    Gorton, L.
    Department of Analytical Chemistry, University of Lund, Lund, Sweden.
    Marko-Varga, G.
    Department of Analytical Chemistry, University of Lund, Lund, Sweden.
    Domínguez, E.
    Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, Alcalá de Henares, Spain.
    Ortega, F.
    Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, Alcalá de Henares, Spain.
    Narváez, A.
    Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, Alcalá de Henares, Spain.
    Irth, H.
    Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden, The Netherlands.
    Lutz, Mareike
    Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden, The Netherlands.
    Puig, D.
    Department of Environmental Chemistry, CID-CSIC, Barcelona, Spain.
    Barceló, D.
    Department of Environmental Chemistry, CID-CSIC, Barcelona, Spain.
    Optimisation and validation of an automated solid phase extraction technique coupled on-line to enzyme-based biosensor detection for the determination of phenolic compounds in surface water samples1995In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 41, no 3-4, p. 207-215Article in journal (Refereed)
    Abstract [en]

    A fully integrated screening system for phenolic compounds was developed incorporating on-line solid phase extraction, fractionation and biosensor detection. Two different types of biosensors, solid graphite and carbon paste electrodes incorporating the enzyme tyrosinase, were compared and used in the screening system. Interfacing of the solid phase extraction and fractionation with the biosensor detection was given special attention since the biosensors were not compatible with the organic modifier used for desorption of phenols from the solid phase extraction step. The system was validated with conventional analytical techniques. Surface water samples from the Ebro river were spiked with 1,10, and 25μg L−1 of catechol, phenol,p-cresol, respectively. Three out of seven samples were spiked and the correct samples were identified, containing phenols equivalent to the spiked concentrations. © 1995 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

  • 2.
    Lutz, Mareike
    et al.
    Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
    Larsson, Marita
    Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
    On-Line Microdialysis-Electrospray Mass Spectrometry for Automated Desalting of Small-Volume Peptide Samples1999In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 49, no Suppl. 1, p. S28-S34Article in journal (Refereed)
    Abstract [en]

    Electrospray ionization mass spectrometry (ESI-MS) is an important tool for biomolecule analysis. Because the salt content of small-volume peptide samples can hamper analyte ionization, such samples require treatment before ESI-MS.The approach described here consists in interfacing ESI-MS with on-line microdialysis which affords rapid desalting and buffer-exchange. On-line microdialysis was performed by means of a hollow fiber (i.d. 200 μm) coupled to fused silica capillaries. Peptide samples were introduced into the capillary flow system as plugs and transferred to the dialysis cell and the electrospray by means of hydrodynamic pressure. As a result, the ionization efficiency of peptidic analytes was increased and adduct formation with, e.g., sodium, was reduced owing to reduced levels of nonvolatile salts. The feasibility of on-line microdialysis-ESI-MS is shown with a proteolytic digest originating from two-dimensional gel electrophoresis.

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