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  • 1.
    Lutz, Mareike
    et al.
    Division of Analytical Chemistry, Leiden/Amsterdam Ctr. for Drug Res., Leiden University, Leiden, Netherlands.
    Oosterkamp, Aaike J.
    Division of Analytical Chemistry, Leiden/Amsterdam Ctr. for Drug Res., Leiden University, Leiden, Netherlands.
    Irth, Hubertus
    Division of Analytical Chemistry, Leiden/Amsterdam Ctr. for Drug Res., Leiden University, Leiden, Netherlands.
    On-line Coupling of Liquid Chromatography to Biological Assays1997In: Chimica oggi, ISSN 0392-839X, E-ISSN 1973-8250, Vol. 15, no 1-2, p. 11-15Article in journal (Refereed)
    Abstract [en]

    Combining two powerful technologies - liquid chromatography and bioassays results in analytical methodologies which are characterised by high selectivity and sensitivity. In contrast to microtitre-type bioassays, biochemical reactions proceed in a closed, continuous-flow reaction detection system coupled directly to the outlet of the chromatographic separation column. The interaction of substances eluting from the separation column with molecular targets such as antibodies or receptors is monitored directly overcoming tedious fraction collection and manual operations required to prepare the functions for batch immunoassays. important application areas are bioanalysis and drug discovery.

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