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A cytogenetic and phenotypic characterization of somatic hybrid plants obtained after fusion of two different dihaploid clones of potato (Solatium tuberosum L.)
Department of Physiological Botany, University of Uppsala, Uppsala, Sweden.ORCID iD: 0000-0003-0553-7918
Rijksuniversiteit Groningen, Department of Genetics, Groningen, Netherlands.
Rijksuniversiteit Groningen, Department of Genetics, Groningen, Netherlands.
Uppsala Universitet, Department of Physiological Botany, Uppsala, Sweden.
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1992 (English)In: Theoretical and Applied Genetics, ISSN 0040-5752, E-ISSN 1432-2242, Vol. 85, no 4, 470-479 p.Article in journal (Refereed) Published
Abstract [en]

Somatic hybrid plants of various ploidy levels obtained after chemical fusion between two dihaploid clones of potato Solanum tuberosum L. have been analysed by cytological, morphological and molecular methods. The hybrid nature of tetraploid and hexaploid plants and the genome dosage in hexaploid hybrids were confirmed by Giemsa C-banding. Tetraploid and hexaploid hybrids showed numerical as well as structural chromosome mutations. The latter occurred mainly in the nuclear organizing chromosome. The tetraploid hybrids were more vigorous than the dihaploid parents as demonstrated by an increase in height, enlargement of leaves, increase in the number of internodes, restored potential for flowering and increased tuber yield. The grouping of tetraploid somatic hybrids into various classes on the basis of leaf morphology revealed that plants with a full chromosome complement were more uniform than aneuploids. Many hexaploid somatic hybrids were also more vigorous than the dihaploidparents and could be grouped into two different classes on the basis of floral colour and tuber characteristics, the differences being due to their different dosage of parental genomes. Most of the tetraploid somatic hybrids showed pollen development halted at the tetrad stage as one of the parental clones contained a S. Stoloniferum cytoplasm. However, one tetraploid plant produced pollen grains with high viability. The chloroplast genome in the hybrid plants was determined by RFLP analysis. All of the hybrids had a cpDNA pattern identical to one parent, which contained either S. Tuberosum or S. Stoloniferum cpDNA. A slight preference for S. Tuberosum plastids were observed in hybrid plants. No correlation between pollen development and plastid type could be detected. © 1992 Springer-Verlag.

Place, publisher, year, edition, pages
New York: Springer-Verlag New York, 1992. Vol. 85, no 4, 470-479 p.
Keyword [en]
cpDNA, Cytology, Morphology, Solanum tuberosum, Somatic hybrids
National Category
Botany
Identifiers
URN: urn:nbn:se:hh:diva-30828DOI: 10.1007/BF00222329ISI: A1992KE52200013PubMedID: 24197462Scopus ID: 2-s2.0-0000323620OAI: oai:DiVA.org:hh-30828DiVA: diva2:925852
Available from: 2016-05-03 Created: 2016-05-03 Last updated: 2017-11-30Bibliographically approved
In thesis
1. Production and characterization of intraspecific somatic hybrids of potato (Solanum tuberosum L.)
Open this publication in new window or tab >>Production and characterization of intraspecific somatic hybrids of potato (Solanum tuberosum L.)
1991 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

A culture protocol has been developed for mesophyll protoplasts isolated from various d¡haploid clones of potato. A large number of call¡ was obtained after serial dilution of the cultures w¡th a suitable amount and type of medium at different stages of cell colony development.

A polyelhylene glycol fusion procedure was developed that yielded up to 12 % heterokaryons. Using this fusion protocol hybrid cells have been manually lsolated, cultured and regenerated into plants. Fus¡on products were identified 2-3 days after fuslon by the dual fluorescence emission from the chloroplasts in mesophyll protoplasts (red) and the fluorescein diacetate stain in l¡ght and norflurazon bleached protoplasts (yellow-green). This fusion and select¡on strategy leads to the recovery of almost 100 % hybr¡d plants as established by isozyme analysls.

Cytolog¡cal analysis of protoplast-derived plants and somatic hybrid plants revealed genetic changes as a consequence of protoplast culture and protoplast fus¡on. Twenty-three tetraplo¡d somatic hybrid plants were obta¡ned from 6 different calli and 9 of these were euploid.

Morphological assessments of somatic hybr¡d plants of different ploidy levels demonstrated that tetraploid as well as several hexaploid somatic hybrids showed an increased vigour as compared to the parental plants. Most tetraploid somat¡c hybrids had a similar appearance although not all euploid plants were identical. Loss of vigour was evident in all mixoploid and octoplo¡d plants. These were stunted, weak and had an abnormal leaf morphology.

The plastid type in hybrid plants was determined by RFLP analysis. All analysed hybrids had a cpDNA restrict¡on fragment pattern ¡dent¡cal to one of the parents wh¡ch contained either S. tuberosum or S. stoloniferum cpDNA. A slight preference for S. tuberosum plastids was observed in hybild plants. No influence on tho assortment of chloroplast by the norflurazon bleaching could be detected.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 1991. 44 p.
Series
Comprehensive summaries of Uppsala dissertations from the Faculty of Science, ISSN 0282-7468 ; 323
National Category
Plant Biotechnology
Identifiers
urn:nbn:se:hh:diva-23705 (URN)91-554-2748-0 (ISBN)
Public defence
1991-05-16, Uppsala, 10:00
Note

Financial support was given by the Swedish Council for Forestry and Agricultural Research.

Available from: 2010-02-23 Created: 2013-10-02 Last updated: 2016-05-03Bibliographically approved

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Waara, Sylvia

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