This study examines how perceptions of role ambiguity, role conflict, team conflict, and cohesion can predict collective efficacy in sports teams. The participants were professional female and male football players, who participated in the First and Second Divisions in Spain. We adopted a longitudinal perspective, taking measures at the beginning, the middle, and the end of a sport season. Multilevel modelling analysis showed that perceptions of team conflict and cohesion, at the interpersonal and interteam levels, can predict changes in collective efficacy. However, individual perceptions of role ambiguity and role conflict were not relevant in establishing a team’s confidence. These results suggest interesting practical applications for coaches and sports psychologists in the professional sphere. © 2015 Elsevier Ltd.