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Influence of chronic and acute spinal cord injury on skeletal muscle Na+-K+-ATPase and phospholemman expression in humans
Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.ORCID iD: 0000-0003-4235-0634
Section for Spinal Cord Injury, Sunnaas Rehabilitation Hospital, Nesoddtangen, Norway & Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
Department of Molecular Medicine and Surgery, Section for Integrative Physiology, Karolinska Institutet, Stockholm, Sweden.
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2012 (English)In: American Journal of Physiology. Endocrinology and Metabolism, ISSN 0193-1849, E-ISSN 1522-1555, Vol. 302, no 7, E864-E871 p.Article in journal (Refereed) Published
Abstract [en]

Na +-K +-ATPase is an integral membrane protein crucial for the maintenance of ion homeostasis and skeletal muscle contractibility. Skeletal muscle Na +-K +-ATPase content displays remarkable plasticity in response to long-term increase in physiological demand, such as exercise training. However, the adaptations in Na +-K +-ATPase function in response to a suddenly decreased and/or habitually low level of physical activity, especially after a spinal cord injury (SCI), are incompletely known. We tested the hypothesis that skeletal muscle content of Na +-K +-ATPase and the associated regulatory proteins from the FXYD family is altered in SCI patients in a manner dependent on the severity of the spinal cord lesion and postinjury level of physical activity. Three different groups were studied: 1) six subjects with chronic complete cervical SCI, 2) seven subjects with acute, complete cervical SCI, and 3) six subjects with acute, incomplete cervical SCI. The individuals in groups 2 and 3 were studied at months 1, 3, and 12 postinjury, whereas individuals with chronic SCI were compared with an able-bodied control group. Chronic complete SCI was associated with a marked decrease in [ 3H]ouabain binding site concentration in skeletal muscle as well as reduced protein content of the α 1-, α 1-, and (β1-subunit of the Na +-K +-ATPase. In line with this finding, expression of the Na +-K +-ATPase α 1-, α 1- subunits progressively decreased during the first year after complete but not after incomplete SCI. The expression of the regulatory protein phospholemman (PLM or FXYD1) was attenuated after complete, but not incomplete, cervical SCI. In contrast, FXYD5 was substantially upregulated in patients with complete SCI. In conclusion, the severity of the spinal cord lesion and the level of postinjury physical activity in patients with SCI are important factors controlling the expression of Na +-K +-ATPase and its regulatory proteins PLM and FXYD5. © 2012 the American Physiological Society.

Place, publisher, year, edition, pages
Bethesda, MD: American Physiological Society , 2012. Vol. 302, no 7, E864-E871 p.
Keyword [en]
FXYD proteins, Paralysis, Physical inactivity, Sodium pump
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:hh:diva-26613DOI: 10.1152/ajpendo.00625.2011ISI: 000302342100012PubMedID: 22275761Scopus ID: 2-s2.0-84859473984OAI: oai:DiVA.org:hh-26613DiVA: diva2:750422
Funder
Swedish Research Council
Note

This work was supported by grants from the Netherlands Organization for Scientific Research, the Throne Holst Foundation of the University of Oslo, the Norwegian South-Eastern Health Authority, the Swedish Research Council, the Novo-Nordisk Foundation, and the Commission of the European Communities (EUGENEHEART and EXGENESIS).

Available from: 2014-09-29 Created: 2014-09-29 Last updated: 2015-09-29Bibliographically approved

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CiteExportLink to record
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