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Significant intramyocellular lipid use during prolonged cycling in endurance-trained males as assessed by three different methodologies
Department of Movement Sciences, Nutrition Research Institute Maastricht, Maastricht University, Maastricht, Netherlands & Department of Movement Science, Maastricht University, Maastricht, Netherlands.
Department of Human Biology, Nutrition Research Institute Maastricht, Maastricht University, Maastricht, Netherlands.ORCID iD: 0000-0003-4235-0634
Department of Movement Sciences, Nutrition Research Institute Maastricht, Maastricht University, Maastricht, Netherlands.
Department of Movement Sciences, Nutrition Research Institute Maastricht, Maastricht University, Maastricht, Netherlands.
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2007 (English)In: American Journal of Physiology. Endocrinology and Metabolism, ISSN 0193-1849, E-ISSN 1522-1555, Vol. 292, no 6, E1715-E1723 p.Article in journal (Refereed) Published
Abstract [en]

Intramyocellular triacylglycerol (IMTG) has been suggested to represent an important substrate source during exercise. In the present study, IMTG utilization during exercise is assessed through the use of various methodologies. In addition, we identified differences in the use of intramyocellular lipids deposited in the immediate subsarcolemmal (SS) area and those stored in the more central region of the fiber. Contemporary stable isotope technology was applied in combination with muscle tissue sampling before and immediately after 3 h of moderate-intensity cycling exercise (62 ± 2% V̇o2 max) in eight well-trained male cyclists. Continuous infusions with [U-13C]palmitate and [6,6-2H2]glucose were applied to quantify plasma free fatty acid (FFA) and glucose oxidation rates and to estimate whole body IMTG and glycogen use. Both immunohistochemical analyses of oil red O (ORO)-stained muscle cross sections and biochemical triacylglycerol (TG) extraction were performed to assess muscle lipid content. During exercise, plasma FFA, muscle (and/or lipoprotein)-derived TG, plasma glucose, and muscle glycogen oxidation contributed 24 ± 2, 22 ± 3, 11 ± 1, and 43 ± 3% to total energy expenditure, respectively. In accordance, a significant net decline in muscle lipid content was observed following exercise as assessed by ORO staining (67 ± 8%) and biochemical TG extraction (49 ± 8%), and a positive correlation was observed between methods (r = 0.56; P < 0.05). Lipid depots located in the SS area were utilized to a greater extent than the more centrally located depots. This is the first study to show significant use of IMTG as a substrate source during exercise in healthy males via the concurrent implementation of three major methodologies. In addition, this study shows differences in resting subcellular intramyocellular lipid deposit distribution and in the subsequent net use of these deposits during exercise. Copyright © 2007 the American Physiological Society.

Place, publisher, year, edition, pages
Bethesda, MD: American Physiological Society , 2007. Vol. 292, no 6, E1715-E1723 p.
Keyword [en]
fat metabolism, intramyocellular triacylglycerol, skeletal muscle, cycling, substrate utilization
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:hh:diva-26621DOI: 10.1152/ajpendo.00678.2006ISI: 000247939100025PubMedID: 17299080Scopus ID: 2-s2.0-34447579088OAI: oai:DiVA.org:hh-26621DiVA: diva2:750412
Available from: 2014-09-29 Created: 2014-09-29 Last updated: 2015-09-29Bibliographically approved

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