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On-Line Microdialysis-Electrospray Mass Spectrometry for Automated Desalting of Small-Volume Peptide Samples
Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
Bioanalytical Chemistry, Astra Hässle AB, Mölndal, Sweden.
1999 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 49, no Suppl. 1, S28-S34 p.Article in journal (Refereed) Published
Abstract [en]

Electrospray ionization mass spectrometry (ESI-MS) is an important tool for biomolecule analysis. Because the salt content of small-volume peptide samples can hamper analyte ionization, such samples require treatment before ESI-MS.The approach described here consists in interfacing ESI-MS with on-line microdialysis which affords rapid desalting and buffer-exchange. On-line microdialysis was performed by means of a hollow fiber (i.d. 200 μm) coupled to fused silica capillaries. Peptide samples were introduced into the capillary flow system as plugs and transferred to the dialysis cell and the electrospray by means of hydrodynamic pressure. As a result, the ionization efficiency of peptidic analytes was increased and adduct formation with, e.g., sodium, was reduced owing to reduced levels of nonvolatile salts. The feasibility of on-line microdialysis-ESI-MS is shown with a proteolytic digest originating from two-dimensional gel electrophoresis.

Place, publisher, year, edition, pages
Wiesbaden: Vieweg , 1999. Vol. 49, no Suppl. 1, S28-S34 p.
Keyword [en]
Electrospray ionization, Mass spectrometric detection, Microdialysis, Peptide analysis
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:hh:diva-24124DOI: 10.1007/BF02468973ISI: 000080256100005Scopus ID: 2-s2.0-0032913175OAI: oai:DiVA.org:hh-24124DiVA: diva2:679536
Conference
Symposium on High-Performance Liquid-Phase Micro Separations, Lund, Sweden, June 7–10, 1998
Available from: 2013-12-16 Created: 2013-12-09 Last updated: 2015-03-03Bibliographically approved

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CiteExportLink to record
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