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Development and Optimization of a Solid Composite Tyrosinase Biosensor for Phenol Detection in Flow Injection Systems
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
Department of Analytical Chemistry, Faculty of Pharmacy, University of Alcalá de Henares, Alcalá de Henares (Madrid), Spain.
1996 (English)In: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 8, no 2, p. 117-123Article in journal (Refereed) Published
Abstract [en]

Bulk-modified epoxy-graphite tyrosinase biosensors were fabricated by four different procedures. The influence of these fabrication procedures on the analytical performance of the enzyme electrode in an amperometric wall-jet flow cell has been studied. The bioprobe performance is assessed by cyclic voltammetry. Higher current densities and narrower peaks were obtained when the enzyme was introduced in the dry state into the epoxy-graphite material, instead of introducing it previously dissolved in the buffer. In the FI system responses of 11.79 μA cm-2 and 1.43 μA cm-2 are then obtained for catechol and phenol respectively for 50 μL injections of 20 μM solutions. Moreover, if gold/palladium is introduced into the epoxy-graphite, a further increase in current is achieved resulting in 27.70 μA cm-2 and 4.90 μA cm-2 for catechol and phenol, respectively. This biosensor can operate in aqueous as well as in mixed aqueous-organic environments.

Place, publisher, year, edition, pages
Weinheim: Wiley-VCH Verlagsgesellschaft, 1996. Vol. 8, no 2, p. 117-123
Keywords [en]
tyrosinase, biocomposite, enzyme electrode, phenolic compounds
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:hh:diva-24130DOI: 10.1002/elan.1140080204ISI: A1996UB42300003Scopus ID: 2-s2.0-1542788440OAI: oai:DiVA.org:hh-24130DiVA, id: diva2:679525
Note

Financial support from the Commission of the European Community, Environment programme 1990-1994 No. EV5V-CT92-0 109 and the Spanish Interministerial Commission of Science and Technology (AMB93-0800-CE) are gratefully acknowledged. Mareike Lutz was a recipient of a grant provided by the Spanish Foreign Office.

Available from: 2013-12-16 Created: 2013-12-09 Last updated: 2018-03-22Bibliographically approved

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