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Effects of different additives on a tyrosinase based carbon paste electrode
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden, Netherlands.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
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1995 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 305, no 1-3, 8-17 p.Article in journal (Refereed) Published
Abstract [en]

The influence of a number of solid and chemical additives on the sensitivity and operational stability of a tyrosinase carbon paste electrode was studied. Cyclic voltammograms were run of the electrochemically active catechol/o-quinone couple on unmodified and additive modified carbon paste electrodes without tyrosinase. This was done in order to study the influence of these additives on the pure electrochemistry of the carbon paste. The influence on the total system (additive and enzyme modified carbon paste electrode) was studied in the flow injection mode. In some instances a dramatic improvement of the direct electron transfer of the catechol/o-quinone couple was obtained with both solid and chemical additives included in the carbon paste. A similar improvement of biosensor sensitivity in the flow injection mode was obtained with most chemical additives whereas the solid additives had a negative impact on biosensor sensitivity. The results obtained in this work indicate that these additives influence the purely electrochemical processes at the carbon paste and/or the performance of the enzyme in the carbon paste environment. How and why these additives can possibly influence the biosensor performance are discussed. © 1995.

Place, publisher, year, edition, pages
Amsterdam: Elsevier, 1995. Vol. 305, no 1-3, 8-17 p.
Keyword [en]
Biosensors, Tyrosinase
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:hh:diva-24131DOI: 10.1016/0003-2670(94)00573-5ISI: A1995QU41900003Scopus ID: 2-s2.0-0028916625OAI: oai:DiVA.org:hh-24131DiVA: diva2:679521
Conference
5th European Conference on Electroanalysis (ESEAC 94), Venice, Italy, May 22-26, 1994
Available from: 2013-12-16 Created: 2013-12-09 Last updated: 2015-03-03Bibliographically approved

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CiteExportLink to record
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Citation style
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