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Optimisation and validation of an automated solid phase extraction technique coupled on-line to enzyme-based biosensor detection for the determination of phenolic compounds in surface water samples
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
Department of Analytical Chemistry, University of Lund, Lund, Sweden.
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1995 (English)In: Chromatographia, ISSN 0009-5893, E-ISSN 1612-1112, Vol. 41, no 3-4, 207-215 p.Article in journal (Refereed) Published
Abstract [en]

A fully integrated screening system for phenolic compounds was developed incorporating on-line solid phase extraction, fractionation and biosensor detection. Two different types of biosensors, solid graphite and carbon paste electrodes incorporating the enzyme tyrosinase, were compared and used in the screening system. Interfacing of the solid phase extraction and fractionation with the biosensor detection was given special attention since the biosensors were not compatible with the organic modifier used for desorption of phenols from the solid phase extraction step. The system was validated with conventional analytical techniques. Surface water samples from the Ebro river were spiked with 1,10, and 25μg L−1 of catechol, phenol,p-cresol, respectively. Three out of seven samples were spiked and the correct samples were identified, containing phenols equivalent to the spiked concentrations. © 1995 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

Place, publisher, year, edition, pages
Heidelberg: Springer Berlin/Heidelberg, 1995. Vol. 41, no 3-4, 207-215 p.
Keyword [en]
Column liquid chromatography, Solid phase extraction, Enzyme-based biosensor detection, Tyrosinase, Phenols
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:hh:diva-24132DOI: 10.1007/BF02267957ISI: A1995RY73400014Scopus ID: 2-s2.0-0029148882OAI: oai:DiVA.org:hh-24132DiVA: diva2:679514
Available from: 2013-12-16 Created: 2013-12-09 Last updated: 2015-03-03Bibliographically approved

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