The purpose of this study was to investigate relationships between motivational profile, self-efficacy, basic needs satisfaction, exercise behavior, and well-being among Swedish university students. A set of the instruments including GLTEQ, SHIS, BPNES, BREQ-2 and BARSE was distributed at a university in southern Sweden. The respondents (n=260) included men (n=122) and women (n=138). For analysis and processing of the gathered data SPSS was used with Pearson’s r and Multiple Regression Analysis. The results showed that competence, autonomy and relatedness were positive predictors of self-determined motivation, whilst identified regulation, intrinsic regulation and barrier self-efficacy were positive predictors for strenuous exercise. Moreover, a regression analysis showed that only competence was a significant predictor for well-being; however, positive correlations were shown between all the basic needs and well-being. Satisfaction of the basic needs seem to result in more self-determined motivation and higher levels of barrier self-efficacy, which in turn increases the number of strenuous exercise sessions per week. Furthermore, satisfaction of the basic needs, especially competence through exercise, appears to be important for university students’ well-being. Strenuous exercise itself may also have the potential to positively influence well-being.