hh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Myogenic reprogramming of bone marrow derived cells in a W⁴¹Dmd(mdx) deficient mouse model
Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
Halmstad University, School of Social and Health Sciences (HOS), Centre of Research on Welfare, Health and Sport (CVHI). (Hälsoinnovationer för barns psykiska hälsa)ORCID iD: 0000-0002-3576-2393
Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
2011 (English)In: PLoS ONE, ISSN 1932-6203, Vol. 6, no 11, e27500- p.Article in journal (Refereed) Published
Abstract [en]

Lack of expression of dystrophin leads to degeneration of muscle fibers and infiltration of connective and adipose tissue. Cell transplantation therapy has been proposed as a treatment for intractable muscle degenerative disorders. Several reports have demonstrated the ability of bone-marrow derived cells (BMDC) to contribute to non-haematopoietic tissues including epithelium, heart, liver, skeletal muscle and brain following transplantation by means of fusion and reprogramming. A key issue is the extent to which fusion and reprogramming can occur in vivo, particularly under conditions of myogenic deterioration.To investigate the therapeutic potential of bone marrow transplantation in monogenetic myopathy, green fluorescent protein-positive (GFP+) bone marrow cells were transplanted into non-irradiated c-kit receptor-deficient (W⁴¹) mdx mice. This model allows BMDC reconstitution in the absence of irradiation induced myeloablation. We provide the first report of BMDC fusion in a W⁴¹Dmd(mdx) deficient mouse model.In the absence of irradiation induced injury, few GFP+ cardiomyocytes and muscle fibres were detected 24 weeks post BMT. It was expected that the frequency of fusion in the hearts of W⁴¹Dmd(mdx) mice would be similar to frequencies observed in infarcted mice. Although, it is clear from this study that individual cardiomyocytes with monogenetic deficiencies can be rescued by fusion, it is as clear that in the absence of irradiation, the formation of stable and reprogrammed fusion hybrids occurs, with the current techniques, at very low levels in non-irradiated recipients.

Place, publisher, year, edition, pages
San Francisco: Public Library of Science , 2011. Vol. 6, no 11, e27500- p.
Keyword [en]
dystrophin, green fluorescent protein, stem cell factor receptor, animal cell, animal experiment, animal model, animal tissue, article, bone marrow cell, bone marrow transplantation, cell fusion, cellular distribution, controlled study, Duchenne muscular dystrophy, gene frequency, heart infarction, heart muscle cell, hematopoietic stem cell transplantation, irradiation, mouse, muscle cell, nonhuman, nuclear reprogramming, protein expression, protein localization, soleus muscle, tibialis anterior muscle, tissue distribution, X chromosome linked muscular dystrophic mouse
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:hh:diva-17454DOI: 10.1371/journal.pone.0027500ISI: 000298163400007PubMedID: 22140444Scopus ID: 2-s2.0-82155181957OAI: oai:DiVA.org:hh-17454DiVA: diva2:513909
Available from: 2012-04-04 Created: 2012-04-04 Last updated: 2015-09-21Bibliographically approved

Open Access in DiVA

fulltext(392 kB)83 downloads
File information
File name FULLTEXT01.pdfFile size 392 kBChecksum SHA-512
9cad79bf77415290eb6bae514d9b360c4f97c734f25b04451deb7c411a9fc6bc3b5ec9ee2347f3a06f122862da774102d33fa4ff7add32533305c269341d2f81
Type fulltextMimetype application/pdf

Other links

Publisher's full textPubMedScopus

Search in DiVA

By author/editor
Nygren, Jens
By organisation
Centre of Research on Welfare, Health and Sport (CVHI)
In the same journal
PLoS ONE
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 83 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 126 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf