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Olf/EBF proteins are expressed in neuroblastoma cells: potential regulators of the Chromogranin A and SCG10 promoters
Department of Laboratory Medicine, Lund University, University Hospital MAS, Malmö, Sweden.
Department of Laboratory Medicine, Lund University, University Hospital MAS, Malmö, Sweden.
Department for Stem Cell Biology, Lund University, Lund, Sweden.
Department for Stem Cell Biology, Lund University, Lund, Sweden.ORCID iD: 0000-0002-3576-2393
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2004 (English)In: International Journal of Cancer, ISSN 0020-7136, E-ISSN 1097-0215, Vol. 110, no 1, 22-30 p.Article in journal (Refereed) Published
Abstract [en]

The childhood malignancy neuroblastoma is derived from developmentally arrested sympathetic nervous system precursor cells. To obtain further insight into the molecular processes involved in the formation of these tumors, we decided to investigate the functional role of Olf/EBF (O/E) transcription factors in human neuroblastoma cells. We here report that O/E-1 and O/E-2 are expressed at variable levels in neuroblastoma cell lines and that O/E proteins could be identified by electrophoretic mobility shift assays. To identify potential neuronal target genes for O/E proteins in neuroblastoma cells we investigated the ability of a set of neuronal promoters to interact with O/E-1 in electrophoretic mobility shift assays. This analysis suggested that the Chromogranin A (CgA) and SCG10 promoters both contained binding sites for O/E-1. O/E-1 was able to activate the CgA promoter in vivo and mutation of the O/E-1 binding site in the CgA promoter reduced the functional activity of the element to about 60% of the wild-type in neuroblastoma cells, supporting the idea that O/E proteins may be involved in the control of the CgA promoter. Furthermore, overexpression of O/E-1 in hippocampal progenitor cells led to neurite outgrowth, indicative of a role for O/E proteins in neuronal differentiation.

Place, publisher, year, edition, pages
Wiley-Liss, Inc. , 2004. Vol. 110, no 1, 22-30 p.
Keyword [en]
Chromogranin A, Differentiation, EBF, Neuroblastoma, Olf, SCG10, Sympathetic nervous system
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:hh:diva-15182DOI: 10.1002/ijc.20092ISI: 000220907100004PubMedID: 15054865Scopus ID: 2-s2.0-1942420706OAI: oai:DiVA.org:hh-15182DiVA: diva2:419620
Available from: 2011-05-27 Created: 2011-05-27 Last updated: 2015-09-21Bibliographically approved

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CiteExportLink to record
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