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Failure of Transplanted Bone Marrow Cells to Adopt a Pancreatic β-Cell Fate
Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
Department of Physiological Sciences, Lund University, Lund, Sweden.
Department of Physiological Sciences, Lund University, Lund, Sweden.
Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.ORCID iD: 0000-0002-3576-2393
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2006 (English)In: Diabetes, ISSN 0012-1797, E-ISSN 1939-327X, Vol. 55, no 2, 290-296 p.Article in journal (Refereed) Published
Abstract [en]

Recent studies in normal mice have suggested that transplanted bone marrow cells can transdifferentiate into pancreatic beta-cells at relatively high efficiency. Herein, adopting the same and alternative approaches to deliver and fate map-transplanted bone marrow cells in the pancreas of normal as well as diabetic mice, we further investigated the potential of bone marrow transplantation as an alternative approach for beta-cell replacement. In contrast to previous studies, transplanted bone marrow cells expressing green fluorescence protein (GFP) under the control of the mouse insulin promoter failed to express GFP in the pancreas of normal as well as diabetic mice. Although bone marrow cells expressing GFP under the ubiquitously expressed beta-actin promoter efficiently engrafted the pancreas of normal and hyperglycemic mice, virtually all expressed CD45 and Mac-1/Gr-1, demonstrating that they adopt a hematopoietic rather than beta-cell fate, a finding further substantiated by the complete absence of GFP(+) cells expressing insulin and the beta-cell transcription factors pancreatic duodenal homeobox factor-1 and homeodomain protein. Thus, transplanted bone marrow cells demonstrated little, if any, capacity to adopt a beta-cell fate.

Place, publisher, year, edition, pages
Alexandria, USA: American Diabetes Association , 2006. Vol. 55, no 2, 290-296 p.
Keyword [en]
hematopoietic stem-cells, insulin-producing cells, glucagon-like peptide-1, in-vivo, purkinje neurons, fusion, mice, muscle, transdifferentiation, hepatocytes
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:hh:diva-15177DOI: 10.2337/diabetes.55.02.06.db05-1212ISI: 000235178400004PubMedID: 16443759Scopus ID: 2-s2.0-33644779820OAI: oai:DiVA.org:hh-15177DiVA: diva2:419615
Available from: 2011-05-27 Created: 2011-05-27 Last updated: 2015-09-21Bibliographically approved

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