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Prolonged cell cycle transit is a defining and developmentally conserved hemopoietic stem cell property
Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.ORCID iD: 0000-0002-3576-2393
Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
Hematopoietic Stem Cell Laboratory, Lund Strategic Research Center for Stem Cell Biology and Cell Therapy, Lund University, Lund, Sweden.
2006 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 177, no 1, 201-208 p.Article in journal (Refereed) Published
Abstract [en]

Adult mouse hemopoietic stem cells (HSCs) are typically quiescent and enter and progress through the cell cycle rarely in steady-state bone marrow, but their rate of proliferation can be dramatically enhanced on demand. We have studied the cell cycle kinetics of HSCs in the developing fetal liver at a stage when they expand extensively. Despite that 100% of fetal liver HSCs divide within a 48-h period, their average cell cycle transit time (10.6 h) is twice that of their downstream progenitors, translating into a prolonged G(1) transit and a period of relative quiescence (G(0)). In agreement with their prolonged G(1) transit when compared with hemopoietic progenitors, competitive transplantation experiments demonstrate that fetal HSCs are highly enriched in G(1) but also functional in S-G(2)-M. This observation combined with experimental data demonstrating that adult HSCs forced to expand ex vivo also sustain a uniquely prolonged cell cycle and G(1) transit, demonstrate at least in part why purified HSCs at any state of development or condition are highly enriched in the G(0)-G(1) phases of the cell cycle. We propose that a uniquely prolonged cell cycle transit is a defining stem cell property, likely to be critical for their maintenance and self-renewal throughout development.

Place, publisher, year, edition, pages
Bethesda, USA: American Association of Immunologists , 2006. Vol. 177, no 1, 201-208 p.
Keyword [en]
animal cell, article, bone marrow, cell cycle G0 phase, cell cycle G1 phase, cell cycle G2 phase, cell cycle S phase, cell renewal, clonogenic assay, controlled study, fetus, fetus liver, hematopoietic stem cell, mouse, nonhuman, priority journal
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:hh:diva-15176ISI: 000238471400028PubMedID: 16785515Scopus ID: 2-s2.0-33745322046OAI: oai:DiVA.org:hh-15176DiVA: diva2:419614
Available from: 2011-05-27 Created: 2011-05-27 Last updated: 2015-09-21Bibliographically approved

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Nygren, Jens Martin
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