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Evaluation of single-chromatography PSI-separation without the use of ultracentrifugation
Halmstad University, School of Business and Engineering (SET).
Halmstad University, School of Business and Engineering (SET).
2006 (English)Independent thesis Basic level (degree of Bachelor)Student thesis
Abstract [en]
A simple method of single-chromatography PhotosystemI (PSI)-separation without the use of ultracentrifugation was evaluated. The thylakoids were treated with the detergent n-decyl-β-D-maltopyranoside (DM), centrifuged and run in a DEAE Fast Flow column. The fractions were tested for PSI/PSII content by measuring PSI/PSII activity using an oxygen electrode and by using gel-electrophoresis. The obtained fractions seem to contain more PSI than PSII. However, this simple method does not seem to give as good separation as methods using double-chromatography.
Place, publisher, year, edition, pages
2006.
Keywords [en]
Chromatography, n-decyl-β-D-maltopyranoside, Photosystem I, Photosystem II, Pisum sativum
Identifiers
URN: urn:nbn:se:hh:diva-13249Local ID: U12338OAI: oai:DiVA.org:hh-13249DiVA, id: diva2:368428
Uppsok
Physics, Chemistry, Mathematics
Note
Denna uppsats kan beställas från arkivet / This paper can be ordered from the archive. Kontakta / Contact: arkivet@hh.seAvailable from: 2010-11-09 Created: 2010-11-09Bibliographically approved

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