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Expression of the lumenal domain of the PsaF subunit of Photosystem I in E.coli
Halmstad University, School of Business and Engineering (SET).
Halmstad University, School of Business and Engineering (SET).
Halmstad University, School of Business and Engineering (SET).
2006 (English)Independent thesis Basic level (degree of Bachelor)Student thesis
Abstract [en]
Photosystem I (PSI) catalyses the transport of electrons through the membrane between plastocyanin on the luminal side and ferrodoxin on the stroma side. Plastocyanin is the electron donor and ferrodoxin is the electron acceptor. PsaF is a transmembrane protein involved in plastocyanin’s docking to PSI. At Gothenburg University there is ongoing studies of plastocyanin and the interaction between plastocyanin and PSI. Up until now they have studied isolated plastocyanin and the electron transfer from plastocyanin to PSI. The PSI complex is too big for NMR and therefore it is interesting to isolate the luminal domain of PsaF in order to perform NMR. To achieve this we constructed clones expressing a shortened version of PsaF only containing the luminal domain of PsaF; this was done through mutagenesis.
Place, publisher, year, edition, pages
2006.
Keyword [en]
Photosystem I, PsaF, Plastocyanin, Electron transport, Gene cloning
Identifiers
URN: urn:nbn:se:hh:diva-13244Local ID: U12333OAI: oai:DiVA.org:hh-13244DiVA: diva2:368423
Uppsok
Physics, Chemistry, Mathematics
Note
Denna uppsats kan beställas från arkivet / This paper can be ordered from the archive. Kontakta / Contact: arkivet@hh.seAvailable from: 2010-11-09 Created: 2010-11-09Bibliographically approved

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