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Chlamydomonas reinhardtii: the model of choice to study mitochondria from unicellular photosynthetic organisms.
Institut für Physiologische Chemie, Ludwig-Maximilians-Universität München, Germany.
Halmstad University, School of Business and Engineering (SET), Biological and Environmental Systems (BLESS), Plant Cell Biology: Energy transduction in plant cells.
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, D.F., México.
2007 (English)In: Methods in Molecular Biology, ISSN 1064-3745, Vol. 372, p. 137-149Article in journal (Other academic) Published
Abstract [en]

Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular division, flagellar biogenesis, and, more recently, mitochondrial function. It has distinct advantages in comparison to higher plants because it is unicellular, haploid, and amenable to tetrad analysis, and its three genomes are subject to specific transformation. It also has the possibility to grow either photoautotrophically or heterotrophically on acetate, making the assembly of the photosynthetic machinery not essential for cell viability. Methods developed allow the isolation of C. reinhardtii mitochondria free of thylakoid contaminants. We review the general procedures used for the biochemical characterization of mitochondria from this green alga.

Place, publisher, year, edition, pages
Clifton, NJ: Humana Press, 2007. Vol. 372, p. 137-149
Keywords [en]
Mitochondria, Blue native polyacrylamide gel electrophoresis, Chlamydomonas reinhardtii, Green alga, Import - Competent mitochondria, OXPHOS
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:hh:diva-1880DOI: 10.1007/978-1-59745-365-3_10PubMedID: 18314723Scopus ID: 2-s2.0-40949153878Local ID: 2082/2275ISBN: 978-1-58829-667-2 ISBN: 978-1-59745-365-3 OAI: oai:DiVA.org:hh-1880DiVA, id: diva2:239098
Note

Ingår i: MitochondriaPractical Protocols, ISBN: 978-1-58829-667-2 (Print) 978-1-59745-365-3 (Online)

Available from: 2008-09-11 Created: 2008-09-11 Last updated: 2018-03-23Bibliographically approved

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Franzén, Lars-Gunnar

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CiteExportLink to record
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Citation style
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