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Cycloheximide resistance conferred by novel mutations in ribosomal protein L41 of Chlamydomonas reinhardtii
Department of Biology, University College London, UK.
Department of Plant Physiology, Botanical Institute, Göteborg University, Sweden.
Halmstad University, School of Business and Engineering (SET), Biological and Environmental Systems (BLESS), Plant Cell Biology: Energy transduction in plant cells.
Department of Biology, University College London, UK.
2001 (English)In: Molecular General Genetics, ISSN 0026-8925, E-ISSN 1432-1874, Vol. 264, no 6, p. 790-795Article in journal (Refereed) Published
Abstract [en]

Although most eukaryotic cells are sensitive to the 80S ribosome inhibitor cycloheximide (CYH), naturally occurring CYH resistance is widespread amongst yeast species. The primary determinant of resistance appears to be a single residue within ribosomal protein L41; resistance is acquired by the substitution of a conserved proline (P-56) by a glutamate residue. We have isolated the L41 gene (RPL41) from the green alga Chlamydomonas and investigated the molecular basis of CYH resistance in various mutant strains. In both the wild-type strain and the mutant act-1, a proline is found at the key position in L41.; However, analysis of six independently isolated act-2 mutants reveals that all have point mutations that replace the proline with either leucine or serine. Of the two changes, the leucine mutation confers significantly higher levels of CYH resistance. This work identifies the ACT-2 locus as RPL41 and provides a possible dominant marker for nuclear transformation of C. reinhardtii.

Place, publisher, year, edition, pages
Berlin: Springer-Verlag , 2001. Vol. 264, no 6, p. 790-795
Keywords [en]
Chlamydomonas, Cycloheximide resistance, Ribosomal protein L41, Intron
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:hh:diva-1878DOI: 10.1007/s004380000368ISI: 000167341100007PubMedID: 11254126Scopus ID: 2-s2.0-0035089265Local ID: 2082/2273OAI: oai:DiVA.org:hh-1878DiVA, id: diva2:239096
Available from: 2008-09-10 Created: 2008-09-10 Last updated: 2018-03-23Bibliographically approved

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Franzén, Lars-Gunnar

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Plant Cell Biology: Energy transduction in plant cells
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