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Experimentally reduced insulin/IGF-1 signaling in adulthood extends lifespan of parents and improves Darwinian fitness of their offspring
Uppsala University, Uppsala, Sweden.ORCID iD: 0000-0001-5602-1933
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2019 (English)In: Evolution Letters, Vol. 3, no 2, p. 207-216Article in journal (Refereed) Published
Abstract [en]

Reduced expression of the insulin/insulin-like nutrient-sensing signalling (IIS) pathway gene daf-2 in adult Caenorhabditis elegans nematode worms increases longevity without affecting fecundity, but the effect of parental lifespan extension on adult offspring is largely unknown. Here we show that reduced IIS signalling in parental generation increases offspring fitness. We used RNA interference (RNAi) to silence daf-2 expression in sexually mature C. elegans hermaphrodites from three different genotypes: N2 wildtype, as well as ppw-1 and rrf-1 mutants that are deficient for RNAi in germline and soma, respectively. Long-lived daf-2 RNAi parents showed normal fecundity as self-fertilizing hermaphrodites and improved late-life reproduction when mated to males. Remarkably, the offspring of daf-2 RNAi parents produced more progeny and had higher Darwinian fitness across all three genotypes. Thus, reduced IIS signalling in adulthood improves offspring quality supporting the emerging view that suboptimally high levels of nutrient-sensing signalling in late-life lie at the heart of ageing.

Place, publisher, year, edition, pages
2019. Vol. 3, no 2, p. 207-216
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Evolutionary Biology
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URN: urn:nbn:se:hh:diva-52645DOI: 10.1002/evl3.108OAI: oai:DiVA.org:hh-52645DiVA, id: diva2:1838015
Available from: 2024-02-15 Created: 2024-02-15 Last updated: 2024-03-07Bibliographically approved

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Lind, Martin I.

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