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Collagen type II-specific monoclonal antibody-induced arthritis in mice: description of the disease and the influence of age, sex, and genes
BMC, Lund University, Lund, Sweden.ORCID iD: 0000-0001-7790-8197
BMC, Lund University, Lund, Sweden.
BMC, Lund University, Lund, Sweden.
2003 (English)In: American Journal of Pathology, ISSN 0002-9440, E-ISSN 1525-2191, Vol. 163, no 5, p. 1827-1837Article in journal (Refereed) Published
Abstract [en]

Transfer of collagen type II (CII)-specific monoclonal antibodies induces an acute form of arthritis (collagen type II antibody-induced arthritis, CAIA) in naive mice. Arthritis was induced using a pair of monoclonal antibodies M2139 and CIIC1, binding to J1 and C1(I) epitopes of CII, respectively. Thereafter, lipopolysaccharide injection was used to increase the incidence and severity of the disease. This model was used to investigate the effect of genes, age, and sex as well as effector cells in the end-stage effector phase of arthritis pathogenesis. Injection of a single monoclonal antibody induced arthritis only after lipopolysaccharide stimulation. CAIA showed differences in disease penetration among the susceptible strains indicating the importance of non-major histocompatibility complex genes on the antibody effector pathway. B-cell-deficient mice were susceptible to CAIA and in some genetic backgrounds B-cell deficiency leads to enhanced arthritis. Histology of the affected paws revealed massive infiltrations of neutrophils along with bone and cartilage erosion, pannus formation, and fibrin deposition. Depletion of neutrophils significantly reduced the incidence and severity of the disease. CAIA susceptibility increased with age. Males were more susceptible than females and estrogen treatment decreased the development of arthritis. We conclude that CAIA is an acute arthritis triggered by antibody binding and neutrophils bypassing immune activation but with many characteristics in common with collagen-induced arthritis.

Place, publisher, year, edition, pages
New York: Elsevier, 2003. Vol. 163, no 5, p. 1827-1837
National Category
Rheumatology and Autoimmunity
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URN: urn:nbn:se:hh:diva-48812DOI: 10.1016/S0002-9440(10)63542-0ISI: 000186148200018PubMedID: 14578183Scopus ID: 2-s2.0-0142213223OAI: oai:DiVA.org:hh-48812DiVA, id: diva2:1719123
Available from: 2022-12-14 Created: 2022-12-14 Last updated: 2023-02-17Bibliographically approved

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Nandakumar, Kutty Selva

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