The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: Structure to function analysisShow others and affiliations
2008 (English)In: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 45, no 8, p. 2196-2204Article in journal (Refereed) Published
Abstract [en]
Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody. © 2007 Elsevier Ltd. All rights reserved.
Place, publisher, year, edition, pages
Oxford: Elsevier, 2008. Vol. 45, no 8, p. 2196-2204
Keywords [en]
Arthritis, Autoantibodies, Collagen type II, Rheumatoid factor
National Category
Immunology in the medical area
Identifiers
URN: urn:nbn:se:hh:diva-48847DOI: 10.1016/j.molimm.2007.12.005ISI: 000254942400008PubMedID: 18241923Scopus ID: 2-s2.0-40349084125OAI: oai:DiVA.org:hh-48847DiVA, id: diva2:1719027
2022-12-142022-12-142023-02-22Bibliographically approved