Tatanan A from the Acorus calamus L. root inhibited dengue virus proliferation and infectionsShow others and affiliations
2018 (English)In: Phytomedicine, ISSN 0944-7113, E-ISSN 1618-095X, Vol. 42, p. 258-267Article in journal (Refereed) Published
Abstract [en]
BACKGROUND: Acorus calamus l. (Acoraceae) is a well-known traditional Chinese medicinal plant, whose root are historically mainly used to treat neurodegenerative diseases, and for cholera treatment. This datum strongly indicates the antimicrobial activity of A. calamus.
PURPOSE: Our goal is to find the active constituents of A. calamus to treat dengue virus (DENV) infections, and to study the effects and mechanisms of these active substances.
METHODS: The root of A. calamus was extracted by ethanol. Mosquito larva C6/36 cells were used for DENV2 replication and transfection host. Mouse kidney fibroblast cells (BHK-21) were used as a host cell to study the infection ability of the virus. DENV2-induced cytopathic effect (CPE) and plaque assay were used to evaluate the inhibitory effect of A. calamus extracts on DENV2 infectivity inhibition. The levels of E and NS1 protein expression were measured by real-time PCR and western blot assays.
RESULTS: 12 compounds were isolated from ethanol extract of A. calamus root, tatanan A showed the best anti-DENV ability among these 12 compounds, which significantly alleviated DENV2-induced CPE and cytotoxicity effects, with an EC50 of 3.9 µM. In addition, RNA replication assay further confirmed the antivirus ability of tatanan A. Time-addition assay showed that tatanan A affected the early stage of viral RNA replication, which in turn inhibited mRNA and protein levels of DENV2.
CONCLUSIONS: These results demonstrated the anti-DENV2 effect of tatanan A, in inhibiting DENV2 RNA replication and infections. In summary, tatanan A was found to be a novel natural DENV inhibitor and a potential candidate for the treatment of DENV infectious disease.
Place, publisher, year, edition, pages
München: Elsevier, 2018. Vol. 42, p. 258-267
Keywords [en]
Acorus calamus l, DENV2 infection, Dengue, Tatanan A
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:hh:diva-48395DOI: 10.1016/j.phymed.2018.03.018ISI: 000429905400029PubMedID: 29655694Scopus ID: 2-s2.0-85044939505OAI: oai:DiVA.org:hh-48395DiVA, id: diva2:1703120
Note
This work was supported by the Natural Science Foundation of China (81603118), Medical Scientific Research Foundation of Guangdong Province (A2016119), Guangdong Provincial Application Technology Research and Development Project (2016B020237005), Educational Department of Jiangxi Province of China (GJJ151075), Natural Science Foundation of Jiangxi Province of China (20161BAB215194) and the Scientific Research project of Jiujiang University (2015LGYB35).
2022-10-122022-10-122022-10-20Bibliographically approved