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Characterization of chronic relapsing antibody mediated arthritis in mice with a mutation in Ncf1 causing reduced oxidative burst
Southern Medical University, Guangzhou, China.
Southern Medical University, Guangzhou, China.
Southern Medical University, Guangzhou, China.
Southern Medical University, Guangzhou, China.ORCID iD: 0000-0001-7790-8197
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2022 (English)In: Molecular Biomedicine, E-ISSN 2662-8651, Vol. 3, no 1, article id 14Article in journal (Refereed) Published
Abstract [en]

Rheumatoid arthritis (RA) is a chronic autoimmune disorder affecting joints with a hallmark of autoantibody production. Mannan-enhanced collagen type II (COL2) antibody induced arthritis (mCAIA) in neutrophil cytosolic factor 1(Ncf1) mutation mouse is a chronic disease model imitating RA in mice. In this study, we characterize the chronic phase of mCAIA in Ncf1 mutated (BQ.Ncf1m1j/m1j) mice. Arthritis was induced by an intravenous injection of anti-COL2 monoclonal antibodies on day 0 followed by intra-peritoneal injections of mannan (from Saccharomyces cerevisiae) on days 3 and 65 in BQ.Ncf1 m1j/m1j and BQ mice. Bone erosion was analysed by computed tomography (CT) and blood cell phenotypes by flow cytometry. Cytokines and anti-COL2 antibodies were analyzed with multiplex bead-based assays. The arthritis in the Ncf1m1j/m1j mice developed with a chronic and relapsing disease course, which was followed for 200 days and bone erosions of articular joints were evaluated. An increased number of circulating CD11b+ Ly6G+ neutrophils were observed during the chronic phase, together with a higher level of G-CSF (granulocyte colony-stimulating factor) and TNF-α. In conclusion, the chronic relapsing arthritis of mCAIA in the Ncf1m1j/m1j mice develop bone erosions associated with a sustained neutrophil type of inflammatory responses.

Place, publisher, year, edition, pages
2022. Vol. 3, no 1, article id 14
Keywords [en]
Anti-COL2 antibody, Chronic phase, Collagen type II induced arthritis, Neutrophil cytosolic factor 1, Rheumatoid arthritis
National Category
Immunology in the medical area
Research subject
Health Innovation
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URN: urn:nbn:se:hh:diva-48308DOI: 10.1186/s43556-022-00076-1OAI: oai:DiVA.org:hh-48308DiVA, id: diva2:1702405
Available from: 2022-10-10 Created: 2022-10-10 Last updated: 2023-03-09Bibliographically approved

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Nandakumar, Kutty Selva

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Nandakumar, Kutty SelvaHolmdahl, Rikard
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