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High-frequency blood flow restricted resistance exercise results in acute and prolonged cellular stress more pronounced in type I than in type II fibers
Department of Sport Science and Physical Education, Faculty of Health and Sport Sciences, University of Agder, Kristiansand, Norway; Norwegian Olympic Federation, Oslo, Norway.
Department of Health and Rehabilitation, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Department of Food and Nutrition, and Sport Science, Center for Health and Performance, University of Gothenburg, Gothenburg, Sweden.
Norwegian Olympic Federation, Oslo, Norway; Department of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway.
Liggins Institute, University of Auckland, Auckland, New Zealand.
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2021 (English)In: Journal of applied physiology, ISSN 8750-7587, E-ISSN 1522-1601, Vol. 131, no 1, p. 643-660Article in journal (Refereed) Published
Abstract [en]

Myocellular stress with high-frequency blood flow restricted resistance exercise (BFRRE) was investigated by measures of heat shock protein (HSP) responses, glycogen content and inflammatory markers. Thirteen participants (24±2 years [mean±SD], 9 males) completed two 5-day-blocks of 7 BFRRE sessions, separated by 10 days. Four sets of unilateral knee extensions to failure at 20% of 1RM were performed. Muscle samples obtained before, 1h after the first session in the first and second block ("Acute1" and "Acute2"), after 3 sessions ("Day4"), during the "Rest Week", and at 3 ("Post3") and 10 days post-intervention ("Post10"), were analyzed for HSP70, αB-crystallin, glycogen (PAS staining), mRNAs, miRNAs, and CD68+ (macrophages) and CD66b+ (neutrophils) cell numbers. αB-crystallin translocated from the cytosolic to the cytoskeletal fraction after Acute1 and Acute2 (p<0.05), and immunostaining revealed larger responses in type I than type II fibers (Acute1, 225±184% vs. 92±81%, respectively, p=0.001). HSP70 was increased in the cytoskeletal fraction at Day4 and Post3, and immunostaining intensities were more elevated in type I than in type II fibers at Day4, (206±84% vs. 72±112%, respectively, p<0.001), during the Rest Week (98±66% vs. 42±79%, p<0.001) and at Post3 (115±82% vs. 28±78%, p=0.003). Glycogen content was reduced in both fiber types; but most pronounced in type I, which did not recover until the Rest Week (-15-29%, p≤0.001). Intramuscular macrophage numbers were increased by ~65% post-intervention, but no changes were observed in muscle neutrophils. We conclude that high-frequency BFRRE with sets performed till failure stresses both fiber types, with type I fibers being most affected. © 2021 the American Physiological Society

Place, publisher, year, edition, pages
Rockville: American Physiological Society , 2021. Vol. 131, no 1, p. 643-660
Keywords [en]
Glycogen staining, Inflammation, Kaatsu, Muscle damage, Stress proteins
National Category
Physiology
Identifiers
URN: urn:nbn:se:hh:diva-45140DOI: 10.1152/japplphysiol.00115.2020PubMedID: 33955259Scopus ID: 2-s2.0-85113674668OAI: oai:DiVA.org:hh-45140DiVA, id: diva2:1574635
Available from: 2021-06-28 Created: 2021-06-28 Last updated: 2021-11-19Bibliographically approved

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