BioMAX – the first macromolecular crystallography beamline at MAX IV Laboratory
Number of Authors: 422020 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 27, no 5, p. 1415-1429Article in journal (Refereed) Published
Abstract [en]
BioMAX is the first macromolecular crystallography beamline at the MAX IV Laboratory 3 GeV storage ring, which is the first operational multi-bend achromat storage ring. Due to the low-emittance storage ring, BioMAX has a parallel, high-intensity X-ray beam, even when focused down to 20 µm × 5 µm using the bendable focusing mirrors. The beam is tunable in the energy range 5–25 keV using the in-vacuum undulator and the horizontally deflecting double-crystal monochromator. BioMAX is equipped with an MD3 diffractometer, an ISARA high-capacity sample changer and an EIGER 16M hybrid pixel detector. Data collection at BioMAX is controlled using the newly developed MXCuBE3 graphical user interface, and sample tracking is handled by ISPyB. The computing infrastructure includes data storage and processing both at MAX IV and the Lund University supercomputing center LUNARC. With state-of-the-art instrumentation, a high degree of automation, a user-friendly control system interface and remote operation, BioMAX provides an excellent facility for most macromolecular crystallography experiments. Serial crystallography using either a high-viscosity extruder injector or the MD3 as a fixed-target scanner is already implemented. The serial crystallography activities at MAX IV Laboratory will be further developed at the microfocus beamline MicroMAX, when it comes into operation in 2022. MicroMAX will have a 1 µm × 1 µm beam focus and a flux up to 1015 photons s−1 with main applications in serial crystallography, room-temperature structure determinations and time-resolved experiments. © 2020 International Union of Crystallography.
Place, publisher, year, edition, pages
Chichester: Wiley-Blackwell, 2020. Vol. 27, no 5, p. 1415-1429
Keywords [en]
beamline, macromolecular crystallography, MBA, micro-focus, serial crystallography, automation, remote operation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:hh:diva-42988DOI: 10.1107/S1600577520008723ISI: 000562741000036PubMedID: 32876619Scopus ID: 2-s2.0-85090284348OAI: oai:DiVA.org:hh-42988DiVA, id: diva2:1461222
Funder
Knut and Alice Wallenberg FoundationSwedish Research Council, 2013-2235; 2018-07152; 2018-06454Vinnova, 2018-04969Swedish Research Council Formas, 2019-02496EU, Horizon 2020, 654220
Note
Funding: BioMAX has been funded by Knut and Alice Wallenberg Foundation and twelve Swedish universities; Research conducted at MAX IV, a Swedish national user facility, is supported by the Swedish Research Council under contracts 2013-2235 and 2018-07152, the Swedish Governmental Agency for Innovation Systems under contract 2018-04969, and Formas under contract 2019-02496; FragMAX is supported by Swedish Research Council under contract 2018-06454; RJF and TU acknowledge support from the European Cluster of Advanced Laser Light Sources (EUCALL) project which has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 654220.
2020-08-262020-08-262020-10-26Bibliographically approved