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Current status and future opportunities for serial crystallography at MAX IV Laboratory
MAX IV Laboratory, Lund University, Lund, Sweden.ORCID iD: 0000-0002-5931-5790
Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.ORCID iD: 0000-0002-8081-181X
MAX IV Laboratory, Lund University, Lund, Sweden.
Halmstad University, School of Information Technology, Halmstad Embedded and Intelligent Systems Research (EIS), Centre for Research on Embedded Systems (CERES).ORCID iD: 0000-0002-0480-4079
Number of Authors: 202020 (English)In: Journal of Synchrotron Radiation, ISSN 0909-0495, E-ISSN 1600-5775, Vol. 27, no Part 5, p. 1095-1102Article in journal (Refereed) Published
Abstract [en]

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports – silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed. © 2020 International Union of Crystallography.

Place, publisher, year, edition, pages
Chichester: Wiley-Blackwell, 2020. Vol. 27, no Part 5, p. 1095-1102
Keywords [en]
serial crystallography, macromolecular crystallography, sample delivery, high-viscosity injectors
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:hh:diva-42987DOI: 10.1107/S1600577520008735ISI: 000562741000001PubMedID: 32876583Scopus ID: 2-s2.0-85090295142OAI: oai:DiVA.org:hh-42987DiVA, id: diva2:1461214
Funder
Vinnova, N 2018 04409Available from: 2020-08-26 Created: 2020-08-26 Last updated: 2020-10-23Bibliographically approved

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Friel, R. J.

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