This study shows that women may be at a disadvantage when signaling that they are “entrepreneurial” to venture capitalists. We demonstrate how gender-based disadvantages may arise from role incongruence in entrepreneurship by analyzing multi-source data from 131 venture capital applications, venture capitalists’ cognitions, and their funding decisions. Our analysis indicates that women who signal an entrepreneurial attitude are more likely to elicit prevention considerations from venture capitalists, whereas men who signal such an attitude are more likely to elicit promotion considerations. We also find that promotion considerations increase the amount of financing, whereas prevention considerations decrease the amount of financing. Our study increases knowledge about the gendered cognitions that underlie implicit bias among investors and knowledge about the effects of regulatory focus on funding outcomes by exploring the interaction between gender and entrepreneurial attitude. © 2019 Elsevier Inc.