hh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Lactotetraosylceramide, a novel glycosphingolipid receptor for Helicobacter pylori, present in human gastric epithelium
Halmstad University, School of Information Technology. Institute of Medical Biochemistry, Göteborg University, P. O. Box 440, SE 405 30 Göteborg, Sweden.
Department of Clinical Microbiology and Immunology, Örebro Medical Centre Hospital, SE 701 85 Örebro, Sweden.
Department of General Surgery, Örebro Medical Centre Hospital, SE 701 85 Örebro, Sweden.
Department of Medical Microbiology, Dermatology, and Infection, University of Lund, SE 223 62 Lund, Sweden.
Show others and affiliations
2002 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 277, no 22, p. 19709-19719Article in journal (Refereed) Published
Abstract [en]

The binding of Helicobacterpylori to glycosphingolipids was examined by binding of 35S-labeled bacteria to glycosphingolipids on thin-layer chromatograms. In addition to previously reported binding specificities, a selective binding to a non-acid tetraglycosylceramide of human meconium was found. This H. pylori binding glycosphingolipid was isolated and, on the basis of mass spectrometry, proton NMR spectroscopy, and degradation studies, were identified as Galβ3GlcNAcβ3-Galβ4Glcβ1Cer (lactotetraosylceramide). When using non-acid glycosphingolipid preparations from human gastric epithelial cells, an identical binding of H. pylori to the tetraglycosylceramide interval was obtained in one of seven samples. Evidence for the presence of lactotetraosylceramide in the binding-active interval was obtained by proton NMR spectroscopy of intact glycosphingolipids and by gas chromatography-electron ionization mass spectrometry of permethylated tetrasaccharides obtained by ceramide glycanase hydrolysis. The lactotetraosylceramide binding property was detected in 65 of 74 H. pylori isolates (88%) Binding of H. pylori to lactotetraosylceramide on thin-layer chromatograms was inhibited by preincubation with lactotetraose but not with lactose. Removal of the terminal galactose of lactotetraosylceramide by galactosidase hydrolysis abolished the binding as did hydrazinolysis of the acetamido group of the N-acetylglucosamine. Therefore, Galβ3GlcNAc is an essential part of the binding epitope.

Place, publisher, year, edition, pages
2002. Vol. 277, no 22, p. 19709-19719
Keywords [en]
Bacteria; Cells; Degradation; Gas chromatography; Hydrolysis; Mass spectrometry; Nuclear magnetic resonance spectroscopy, Thin-layer chromatograms, Lipids, galactose; galactosidase; glucan synthase; glycosphingolipid; lactose; lactotetraosylceramide; n acetylglucosamine; sulfur 35; tetrose; unclassified drug, article; autoradiography; bacterium adherence; controlled study; gas chromatography; Helicobacter pylori; human; human tissue; mass spectrometry; meconium; molecular model; priority journal; proton nuclear magnetic resonance; stomach epithelium; thin layer chromatography, Chromatography, Gas; Chromatography, Thin Layer; Epithelium; Epitopes; Galactose; Gastric Mucosa; Glycoside Hydrolases; Glycosphingolipids; Helicobacter pylori; Humans; Lactose; Magnetic Resonance Spectroscopy; Mass Spectrometry; Meconium; Models, Chemical; Models, Molecular; Oligosaccharides; Polysaccharides; Protein Binding; Protein Structure, Tertiary; Temperature; Thermodynamics, Bacteria (microorganisms); Helicobacter; Helicobacter pylori; Negibacteria
National Category
Biochemistry and Molecular Biology Other Basic Medicine Microbiology in the medical area
Identifiers
URN: urn:nbn:se:hh:diva-37948DOI: 10.1074/jbc.M201113200Scopus ID: 2-s2.0-0037205524OAI: oai:DiVA.org:hh-37948DiVA, id: diva2:1248636
Available from: 2018-09-17 Created: 2018-09-17 Last updated: 2018-09-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus
By organisation
School of Information Technology
In the same journal
Journal of Biological Chemistry
Biochemistry and Molecular BiologyOther Basic MedicineMicrobiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf