During the last decades, an increased drop-out rate in adolescents’ team sport participation is observed. Given the potential adverse consequences of drop-out from team sport more information about risk factors for drop-out is warranted. The objectives of this systematic review were to (1) synthesise the literature on factors associated with future drop-out from team sport among adolescents and (2) investigate the strength of associations between drop-out and related factors with meta-analysis. The databases Academic Search Elite, ERIC, PsycINFO, PubMed and SPORTDiscus were searched for relevant publications from the earliest reported date until October 8, 2021. Articles were included if: (1) data about drop-out was collected; (2) the focus was on adolescents; (3) the context was team sport and (4) studies were of prospective design. We used the Risk of Bias Assessment Tool for Non-randomized Studies (RoBANS) to assess the risk of bias in included studies. A narrative synthesis was conducted according to the reporting guideline of synthesis without meta-analysis. Studies that presented statistical data necessary for the calculation of Hedge’s g effect sizes were included in the meta-analysis. In total, 16 studies met the inclusion criteria and were included in the narrative synthesis. The meta-analysis included 12 of the studies. Altogether, 6304 adolescent team sport players participated in the selected studies. Of those studies, most had a focus on intrapersonal factors relationship with drop-out. The results showed that constructs related to motivation as well as sport experience had the strongest relationships with drop-out. To prevent drop-out from adolescents’ team sport, organisations and clubs are recommended to focus on developing a high-quality motivation climate that facilitates motivation and enjoyment. © 2022 The Authors