hh.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Short-term exercise affects cardiac function ex vivo partially via changes in calcium channel levels, without influencing hypoxia sensitivity
University of Turku, Turku, Finland.ORCID iD: 0000-0002-4424-554X
University of Turku, Turku, Finland.ORCID iD: 0000-0002-8785-1216
University of Turku, Turku, Finland; Poznan University of Medical Sciences, Poznan, Poland.
Halmstad University, School of Business, Innovation and Sustainability, The Rydberg Laboratory for Applied Sciences (RLAS). Turku University Hospital, Turku, Finland.ORCID iD: 0000-0001-8608-4839
Show others and affiliations
2021 (English)In: Journal of physiology and biochemistry, ISSN 1138-7548, E-ISSN 1877-8755, Vol. 77, no 4, p. 639-651Article in journal (Refereed) Published
Abstract [en]

Exercise is known to improve cardiac recovery following coronary occlusion. However, whether short-term exercise can improve cardiac function and hypoxia tolerance ex vivo independent of reperfusion injury and the possible role of calcium channels in improved hypoxia tolerance remains unknown. Therefore, in the current study, heart function was measured ex vivo using the Langendorff method at different oxygen levels after a 4-week voluntary wheel-running regimen in trained and untrained male mice (C57Bl/6NCrl). The levels of cardiac Ca2+-channels: L-type Ca2+-channel (CACNA1C), ryanodine receptor (RyR-2), sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA2), and sodium-calcium exchanger were measured using western blot. Trained mice displayed lower cardiac afterload pressure generation capacity (rate and amplitude), but unaltered hypoxia tolerance when compared to untrained mice with similar heart rates. The level of CACNA1C positively correlated with the pressure generation rate and amplitude. Furthermore, the CACNA1C-RYR-2 ratio also positively correlated with the pressure generation rate. While the 4-week training period was not enough to alter the intrinsic cardiac hypoxia tolerance, interestingly it decreased pressure generation capacity and slowed pressure decreasing capacity in the mouse hearts ex vivo. This reduction in pressure generation rate could be linked to the level of channel proteins in sarcolemmal Ca2+-cycling in trained mice. However, the Ca2+-channel levels did not differ significantly between the groups, and thus, the level of calcium channels cannot fully explain all the functional alterations, despite the detected correlations. Therefore, additional studies are warranted to reveal further mechanisms that contribute to the reduced intrinsic capacity for pressure production in trained mouse hearts. © 2021, The Author(s).

Place, publisher, year, edition, pages
Dordrecht: Springer Netherlands, 2021. Vol. 77, no 4, p. 639-651
Keywords [en]
CACNA, Ex vivo, Heart function, Langendorff, NCX, Pressure generation, RyR, SERCA, Training
National Category
Endocrinology and Diabetes
Identifiers
URN: urn:nbn:se:hh:diva-46030DOI: 10.1007/s13105-021-00830-zISI: 000690373400001PubMedID: 34449060Scopus ID: 2-s2.0-85113705022OAI: oai:DiVA.org:hh-46030DiVA, id: diva2:1618452
Available from: 2021-12-09 Created: 2021-12-09 Last updated: 2021-12-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Heinonen, Ilkka

Search in DiVA

By author/editor
Uurasmaa, Tytti-MariaStreng, TomiHeinonen, IlkkaAnttila, Katja
By organisation
The Rydberg Laboratory for Applied Sciences (RLAS)
In the same journal
Journal of physiology and biochemistry
Endocrinology and Diabetes

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 50 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf