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Effects of Different Exercise Training Protocols on Gene Expression of Rac1 and PAK1 in Healthy Rat Fast- and Slow-Type Muscles.
urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland.
Turku Centre for Biotechnology, University of Turku, Åbo Akademi University, Turku, Finland; Natural Resources Institute Finland (Luke), Jokioinen, Finland.
Turku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland; Institute of Biomedicine, University of Turku, Turku, Finland; TuDMM Doctoral Programmes, University of Turku, Turku, Finland.
urku PET Centre, Turku University Hospital, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland.
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2020 (English)In: Frontiers in Physiology, E-ISSN 1664-042X, Vol. 11, article id 584661Article in journal (Refereed) Published
Abstract [en]

Purpose: Rac1 and its downstream target PAK1 are novel regulators of insulin and exercise-induced glucose uptake in skeletal muscle. However, it is not yet understood how different training intensities affect the expression of these proteins. Therefore, we studied the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on Rac1 and PAK1 expression in fast-type (gastrocnemius, GC) and slow-type (soleus, SOL) muscles in rats after HIIT and MICT swimming exercises.

Methods: The mRNA expression was determined using qPCR and protein expression levels with reverse-phase protein microarray (RPPA).

Results: HIIT significantly decreased Rac1 mRNA expression in GC compared to MICT (p = 0.003) and to the control group (CON) (p = 0.001). At the protein level Rac1 was increased in GC in both training groups, but only the difference between HIIT and CON was significant (p = 0.02). HIIT caused significant decrease of PAK1 mRNA expression in GC compared to MICT (p = 0.007) and to CON (p = 0.001). At the protein level, HIIT increased PAK1 expression in GC compared to MICT and CON (by ∼17%), but the difference was not statistically significant (p = 0.3, p = 0.2, respectively). There were no significant differences in the Rac1 or PAK1 expression in SOL between the groups.

Conclusion: Our results indicate that HIIT, but not MICT, decreases Rac1 and PAK1 mRNA expression and increases the protein expression of especially Rac1 but only in fast-type muscle. These exercise training findings may reveal new therapeutic targets to treat patients with metabolic diseases.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2020. Vol. 11, article id 584661
Keywords [en]
HIIT, MICT, PAK1, RPPA, Rac1, exercise, glucose uptake, skeletal muscle
National Category
Physiology
Identifiers
URN: urn:nbn:se:hh:diva-44097DOI: 10.3389/fphys.2020.584661ISI: 000595892200001PubMedID: 33329033Scopus ID: 2-s2.0-85097281747OAI: oai:DiVA.org:hh-44097DiVA, id: diva2:1541875
Funder
Academy of Finland, 251399Academy of Finland, 256470Academy of Finland, 281440Academy of Finland, 283319Academy of Finland, 329001Academy of Finland, 324243Available from: 2021-04-05 Created: 2021-04-05 Last updated: 2024-01-17Bibliographically approved

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Heinonen, Ilkka

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