hh.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Applying hollow fibres for separating free and bound label in continuous-flow immunochemical detection
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
1996 (Engelska)Ingår i: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 755, nr 2, s. 179-187Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

On-line liquid chromatography-immunochemical detection (LC-ICD) provides the possibility to individually monitor cross-reactive compounds overcoming the need of tedious fraction collection. ICD is performed as a post-column reaction detection system and is based on a two-step immunoreaction. In the first step unlabelled antibodies are added to the LC effluent and allowed to react with antigens (analytes) eluting from the LC column. The amount of analytes bound to the antibodies is measured by adding, in a second step, labelled antigen to the reaction mixture. For quantitation, free and bound label need to be separated prior to detection. The present paper describes a hollow fibre module (HFM), which can be used for this purpose. Separation of free and bound label occurs on discrimination by size. Using biotin as a model compound, a detection limit of 30 nmol/l can be reached employing anti-biotin antibodies and a low-molecular-mass fluorescence label in the LC-ICD system. Additional to low-molecular-mass labels, the HFM allows the use of small enzyme labels. In this context, horseradish peroxidase-labelled biotin was used as a label in combination with antibodies in the immunochemical detection of biotin. This allows future implementation of commercially available enzyme immunoassay kits in continuous-flow immunochemical detection.

Ort, förlag, år, upplaga, sidor
Amsterdam: Elsevier B.V , 1996. Vol. 755, nr 2, s. 179-187
Nyckelord [en]
Immunochemical detection, Detection (LC), Hollow fibres, Membranes, Biotin
Nationell ämneskategori
Analytisk kemi
Identifikatorer
URN: urn:nbn:se:hh:diva-24129DOI: 10.1016/S0021-9673(96)00620-6ISI: A1996WA19300003PubMedID: 8997748Scopus ID: 2-s2.0-0030572984OAI: oai:DiVA.org:hh-24129DiVA, id: diva2:679526
Anmärkning

This work was financially supported by the EC-project EC No EV5V-CT92-0109. Gilson Medical Electronics, Villiers-le-Bel, France, is acknowledged for instrumental support. The gift of hollow fibres from X-Flow BV, Almelo, Netherlands, is appreciated.

Tillgänglig från: 2013-12-16 Skapad: 2013-12-09 Senast uppdaterad: 2018-03-22Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Lutz, Mareike

Sök vidare i DiVA

Av författaren/redaktören
Lutz, Mareike
I samma tidskrift
Journal of Chromatography A
Analytisk kemi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 144 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf