Evaluation of single-chromatography PSI-separation without the use of ultracentrifugation 2006 (Engelska)Självständigt arbete på grundnivå (kandidatexamen)Studentuppsats (Examensarbete)

Abstract [en]

A simple method of single-chromatography PhotosystemI (PSI)-separation without the use of ultracentrifugation was evaluated. The thylakoids were treated with the detergent n-decyl-β-D-maltopyranoside (DM), centrifuged and run in a DEAE Fast Flow column. The fractions were tested for PSI/PSII content by measuring PSI/PSII activity using an oxygen electrode and by using gel-electrophoresis. The obtained fractions seem to contain more PSI than PSII. However, this simple method does not seem to give as good separation as methods using double-chromatography.

Ort, förlag, år, upplaga, sidor

2006.

Nyckelord [en]

Chromatography, n-decyl-β-D-maltopyranoside, Photosystem I, Photosystem II, Pisum sativum

Identifikatorer

URN: urn:nbn:se:hh:diva-13249Lokalt ID: U12338OAI: oai:DiVA.org:hh-13249DiVA, id: diva2:368428

Uppsök

fysik/kemi/matematik

Anmärkning

Denna uppsats kan beställas från arkivet / This paper can be ordered from the archive. Kontakta / Contact: arkivet@hh.seTillgänglig från: 2010-11-09 Skapad: 2010-11-09Bibliografiskt granskad

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