hh.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Implementation of affinity solid-phases in continuous-flow biochemical detection
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, Netherlands.
Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, Netherlands.
1997 (engelsk)Inngår i: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 776, nr 2, s. 169-178Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A continuous-flow biochemical detection system is presented which allows the use of solid-phase immobilised affinity proteins. The biochemical detection is performed by mixing analyte with a labelled ligand followed by the addition of solid-phase immobilised affinity protein. After a reaction time of 85 s, free and bound label are separated by means of a hollow fibre module. Quantitation of the free label is performed with a conventional flow-through fluorescence detector. Total assay time amounts to less than 2 min. Biotin was chosen as the model compound using a range of streptavidin-coated solid-phases and an antibody-coated solid-phase as affinity material, and fluorescein–biotin as low-molecular-mass label. The relative standard deviation for twenty repetitive injections was 10.9%. A calibration curve was constructed in the concentration range between 20 and 400 nmol l−1 leading to a correlation coefficient of 0.994. A limit of detection of 8 nmol l−1 was obtained. © 1997 Elsevier Science B.V.

sted, utgiver, år, opplag, sider
Amsterdam: Elsevier B.V , 1997. Vol. 776, nr 2, s. 169-178
Emneord [en]
Detection (LC), Affinity-based detection, Fluorescein, Biotin, Streptavidin
HSV kategori
Identifikatorer
URN: urn:nbn:se:hh:diva-24127DOI: 10.1016/S0021-9673(97)00354-3ISI: A1997XT07000001Scopus ID: 2-s2.0-0030859255OAI: oai:DiVA.org:hh-24127DiVA, id: diva2:679532
Tilgjengelig fra: 2013-12-16 Laget: 2013-12-09 Sist oppdatert: 2018-03-22bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstScopus

Personposter BETA

Lutz, Mareike

Søk i DiVA

Av forfatter/redaktør
Lutz, Mareike
I samme tidsskrift
Journal of Chromatography A

Søk utenfor DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric

doi
urn-nbn
Totalt: 133 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf