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Stenberg, Manne
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Stenberg, M. & Nilsson, K. (1997). Fast tracking of point-symmetric objects. In: Divyendu Sinha (Ed.), Real-time imaging II: 10 February, 1997, San Jose, California. Paper presented at 2nd IS&T/SPIE Conference on Real-Time Imaging, San Jose, CA, USA, 10 February 1997 (pp. 38-47). Bellingham, Wash.: SPIE - International Society for Optical Engineering, 3028
Open this publication in new window or tab >>Fast tracking of point-symmetric objects
1997 (English)In: Real-time imaging II: 10 February, 1997, San Jose, California / [ed] Divyendu Sinha, Bellingham, Wash.: SPIE - International Society for Optical Engineering, 1997, Vol. 3028, p. 38-47Conference paper, Published paper (Refereed)
Abstract [en]

A point-symmetry function based on autoconvolution is described which makes it possible to track the position of point-symmetric objects with sub-pixel accuracy. The method is insensitive to grey level and was developed in order to have a fast and robust algorithm for real-time tracking of small magnetic particles in a light microscopc. The phase contrast microscope image of the 4.5 mu m diameter spherical particle consisted of concentric light and dark fringes where the shape of the fringes were dependent on the focus. The position of the particle could be monitored in real-time at 25 Hz with a lateral accuracy of +/- 20 nm corresponding to less than +/- 0.1 pixel. To determine the vertical or z-position a new parameter was defined representing a measure of the second derivative of the intensity function. The vertical position could thus be determined with an accuracy of +/-50 nm. The magnetic particle could be tracked acid guided by applied magnetic fields to remain in a fixed position or programmed to scan either horizontal or vertical surfaces. Forces down to 10(-14) N could be measured by monitoring the applied magnetic forces. One and two-dimensional Brownian motion could be studied by regulating the particle to a fixed z-position and monitoring the lateral position.

Place, publisher, year, edition, pages
Bellingham, Wash.: SPIE - International Society for Optical Engineering, 1997
Series
Proceedings of SPIE - The International Society for Optical Engineering, ISSN 0277-786X ; 3028
Keywords
tracking, point-symmetry, autoconvolution, video microscopy, Brownian motion
National Category
Engineering and Technology Embedded Systems
Identifiers
urn:nbn:se:hh:diva-18814 (URN)10.1117/12.270349 (DOI)A1997BH60Y00004 ()2-s2.0-0031334559 (Scopus ID)0819424390 (ISBN)
Conference
2nd IS&T/SPIE Conference on Real-Time Imaging, San Jose, CA, USA, 10 February 1997
Available from: 2012-08-16 Created: 2012-06-25 Last updated: 2018-03-22Bibliographically approved
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